Composite

Part:BBa_K3389003:Design

Designed by: C. Curtin, B. Cordice-Little, A. Germosen Rosa, H. Sanchez, M. Hammes, A. Ibrahim, S. Walis, L. Buchanan, C. Tarlen, E. Lopez   Group: iGEM20_SUNY_Oneonta   (2020-10-23)


RAD27 Flappase expression construct with 10x His tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 311
    Illegal BglII site found at 704
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 216


Design Notes

This part was optimized for inducible expression in Escherichia coli and affinity purification of the expressed protein. The sequence includes a strong RBS, T7 promoter, and a 10x His tag.


Source

The source of the RAD27 sequence is the genomic sequence from Saccharomyces cerevisiae, the RAD27 gene (GenBank accession number: NM_001179679.1, part number BBa_K338902).

References